TY  - THES
T1  - Using genome-wide association studies to map genes for complex traits in porcine F2 crosses
A1  - Schmid,Markus
Y1  - 2018/10/22
N2  - In the era of genomics, genome-wide association studies (GWASs) have become the method of choice for gene mapping. This is still of great interest to infer the genetic architecture of quantitative traits and to improve genomic selection in animal breeding. Formerly, linkage analyses were conducted in order to map genes. Therefore, many F2 cross populations were generated by crossing genetically divergent lineages in order to create informative experimental populations. However, a small number of markers and the limited meiotic divisions led to imprecise mapping results. The main objective of the present study was to investigate the use of existing porcine F2 cross data, extended towards single nucleotide polymorphism (SNP) chip genotype information, for quantitative trait loci (QTL) mapping in the genomic era. A special focus was on mapping genes that also segregate within the Piétrain breed since this is an important sire line and genomic selection is applied in this breed.
Chapter 1 is a review article of statistical models and experimental populations applied in GWASs. This chapter gives an overview of methods to conduct GWASs using single-marker models and multi-marker models. Further, approaches taking non-additive genetic effects or genotype-by-environment interactions into account are described. Finally, post-GWAS analysis possibilities and GWAS mapping populations are discussed.
In chapter 2, the power and precision of GWASs in different F2 populations and a segregating population was investigated using simulated whole-genome sequence data. Further, the effect of pooling data was determined. GWASs were conducted for simulated traits with a heritability of 0.5 in F2 populations derived from closely and distantly related simulated founder breeds, their pooled datasets, and a sample of the common maternal founder breed. The study showed that the mapping power was high (low) in F2 crosses derived from distantly (closely) related founder breeds and highest when several F2 datasets were pooled. By contrast, a low precision was observed in the cross with distantly related founder breeds and the pooling of data led to a precision that was between the two crosses. For genes that also segregated within the common founder breed, the precision was generally elevated and, at equal sample size, the power to map QTL was even higher in F2 crosses derived from closely related founder breeds compared with the founder breed itself.
Within and across linkage disequilibrium (LD) structures of such F2 populations were examined in chapter 3 by separately and jointly (pooled dataset) analyzing four F2 datasets generated from different founder breeds. All individuals were genotyped with a 62k SNP chip. The LD decay was faster in crosses derived from closely related founder breeds compared with crosses from phylogenetically distantly related founder populations and fastest when the data of all crosses were pooled. The pooled dataset was also used to map QTL for the economically important traits dressing out and conductivity applying single-marker and Bayesian multi-marker regressions. For these traits, several genome-wide significant association signals were mapped.
To infer the suitability of F2 data to map genes in a segregating breeding population, GWAS results of a pooled F2 cross were validated in two samples of the German Piétrain population (chapter 4). All individuals were genotyped using standard 62k SNP chips. The pooled cross contained the data of two F2 crosses, both had Piétrain as one founder breed, and consisted of 595 individuals. Initially, GWASs were conducted in the pooled F2 cross for the production traits dressing yield, carcass length, daily gain and drip loss. Subsequently, QTL core regions around significant trait associated peaks were defined. Finally, SNPs within these core regions were tested for association in the two samples of the current Piétrain population (771 progeny tested boars and 210 sows) in order to validate them in this breed. In total, 15 QTL were mapped and 8 (5) of them were validated in the boar (sow) validation dataset. This approach takes advantage of the high mapping power in F2 data to detect QTL that may not be found in the segregating Piétrain population. The findings showed that many of the QTL mapped in F2 crosses derived from Piétrain still segregate in this breed, and thus, these F2 datasets provide a promising database to map QTL in the Piétrain breed. 
The thesis ends with a general discussion.

KW  - Genetik
KW  - Tierzucht
KW  - Genkartierung
KW  - Schwein
KW  - Power
KW  - Genauigkeit
KW  - Merkmal
CY  - Hohenheim
PB  - Kommunikations-, Informations- und Medienzentrum der Universität Hohenheim
AD  - Garbenstr. 15, 70593 Stuttgart
UR  - http://opus.uni-hohenheim.de/volltexte/2018/1524
ER  -